Deoxyribonuclease II, or Acid DNase, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates.
As the name implies, it is more effective at acid pHs.
There are several known DNases II, including:DNase II alpha (usually just called DNase II) ;DNase II beta (also called DLAD, or DNase II-Like Acid DNase)The predicted 360aa protein has 3 parts: a 16aa signal peptide, a 91aa propeptide, and a 253aa mature protein region.
like other lysosomal enzymes, DNase II is processed by release of a signal peptide followed by proteolytic processing that generates a 2-chain enzyme.
Purified DNase II migrates as 2 bands (32 and 12 kD) on SDS-PAGE.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human DNASEII.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Cys19-Ile360 of human DNASEII, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
