The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.
The predicted 685aa DLL4 protein exhibits all of the hallmarks of the Delta family of Notch ligands.
It has an extracellular region containing 8 EGF-like repeats and a DSL domain involved in Notch binding, as well as a transmembrane domain and a cytoplasmic tail that lacks catalytic motifs.
Human DLL4 shares 87%aa sequence identity with mouse Dll4.
Northern blot analysis of mouse tissues detected highest Dll4 expression in lung, followed by heart, kidney, skeletal muscle, and brain; Dll4 expression was barely detectable in spleen and testis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat dLL4.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Gly215-Ser331 of rat dLL4, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
