DKK3 is a protein that is a member of the dickkopf family.
The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway.
The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene.
Alternative splicing results in multiple transcript variants encoding the same protein.
DKK3 has 4 potential N-glycosylation sites and possesses an extended N-terminal domain preceding the first cys domain as well as an extended C-terminal region following the second cys domain.
All DKKs have several potential sites for cleavage by furin-type proteases.
Northern blot analysis revealed wide expression of a 2.5-kb DKK3 transcript, with highest expression in heart, brain, and spinal cord.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat DKK3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Pro41-Ile348 of rat DKK3, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
