Dihydroorotate dehydrogenase (EC 1.3.3.1) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.
Minet et al.
(1992) isolated a truncated human cDNA encoding this enzyme from a HeLa cell cDNA library by functional complementation of a corresponding deletion mutant from the Saccharomyces cerevisiae.
The gene in yeast is symbolized ura1.
DHO dehydrogenase is a monofunctional protein which, in most eukyeastaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.
In, however, it has a cytosolic location.
Missense mutations of the DHODH gene cause Miller syndrome, an autosomal recessive disorder also known as postaxial acrofacial dysostosis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human DHODH.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Arg35-Asp392 of human DHODH, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
