Diacylglycerol kinase zeta is an enzyme belongs to the eukaryotic diacylglycerol kinase family.
It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction.
Alternative splicing occurs at this locus and three transcript variants encoding three distinct isoforms have been identified.
Human DGK-zeta contains 2 zinc fingers, an ATP-binding site, and 4 ankyrin repeats near the C terminus.
A unique feature, as compared with other DGKs, is the presence of a sequence homologous to the MARCKS phosphorylation site domain.
Northern blot analysis of multiple tissues showed that DGK-zeta is expressed at highest levels in brain and muscle.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse DGKz.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Tyr657-Val879 of mouse DGKz, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
