Diacylglycerol kinase alpha is an enzyme belongs to the eukaryotic diacylglycerol kinase family.
It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways.
It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid.
Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
Diacylglycerol (DAG) functions in intracellular signaling pathways as an allosteric activator of protein kinase C (PKC).
In addition, DAG appears to play a role in regulating RAS and RHO family proteins by activating the guanine nucleotide exchange factors VAV and RASGRP1.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse DGKa.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to His305-Arg531 of mouse DGKa, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
