Deoxycholic acid is one of the secondary bile acids, which are metabolic byproducts of intestinal bacteria.
The two primary bile acids secreted by the liver are cholic acid and chenodeoxycholic acid.
Bacteria metabolize chenodeoxycholic acid into the secondary bile acid lithocholic acid, and they metabolize cholic acid into deoxycholic acid.
There are additional secondary bile acids, such as ursodeoxycholic acid.
Deoxycholic acid is soluble in alcohol and acetic acid.
When pure, it comes in a white to off-white crystalline powder form.
Deoxycholic acid has been used since its discovery in various fields of human medicine.
In the human body deoxycholic acid is used in the emulsification of fats for the absorption in the intestine.
It has, in some countries been licensed as an emulsifier in food industry, but it is no longer common.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Specificity：Recognizes Deoxycholate. Species Crossreactivity: pan-species (general)
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Immunogen：Small Molecule, Deoxycholate conjugated to OVA.
