Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils.
Members of the defensin family are highly similar in protein sequence.
Beta-defensin 103 has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense.Highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue.
Low expression in salivary gland, bone marrow, colon, stomach, polyp and larynx.
No expression in small intestine.
Exhibits antimicrobial activity against Gram-positive bacteria S.
Aureus and S.pyogenes, Gram-negative bacteria P.
Aeruginosa and E.coli and the yeast C.
Albicans.
Kills multiresistant S.
Aureus and vancomycin-resistent E.faecium.
No significant hemolytic activity was observed.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GIINTLQK YYCRVRGGRC AVLSCLPKEE QIGKCSTRGR KCCRRKK
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human DEFb103A.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly23-Lys67 of human DEFb103A, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
