Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense.
They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine.
This gene differs from other genes of this family by an extra 83-base segment that is apparently the result of a recent duplication within the coding region.
DEFa4 is found in the neutrophils; it exhibits corticostatic activity and inhibits corticotropin stimulated corticosterone production.
The nucleotide sequence shares about 72% identity with the cDNA encoding defensin HP-1, but differed from it and from other genes of this family by an extra 83-base segment.
This extra segment was not adjacent to an intron and was apparently the result of a recent duplication within the coding region corresponding to most of the mature HP-4 peptide.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse DEFa4.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Asp20-Arg92 of mouse DEFa4, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
