DEFa2 belongs to the alpha-defensin family, Has broad-spectrum antimicrobial properties.
Has antibacterial activity against the Gram-positive bacterium L.
monocytogenes EGD and the Gram-negative bacteria E.
coli ML-35p and avirulent S.
Typhimurium 7953, but not against the mouse-virulent S.
Typhimurium 14028S.
DEFA1 and DEFA3 differ only by the first aa of the mature peptides and DEFA2 lacks this residue.
Given that no gene for DEFA2 has been discovered, it is thought that DEFA2 is a proteolytic product of one or both of DEFA1 and DEFA3.
DEFA1 and DEFA2 are active against Candida albicans and are chemotactic for T-cells whereas DEFA3 is not.
DEFA1 and DEFA2 have recently been observed to be more potent than DEFA3 against the Gram negative bacteria Enterobacter aerogenes and Escherichia coli as well as the Gram positive Staphylococcus aureus and Bacillus cereus.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human DEFa2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu20-Cys94 of human DEFa2, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
