The DDIT3 gene shows a high level of conservation in both the structural and regulatory regions to the previously described hamster gene.
The human and hamster proteins share 91% aa identity.
CHOP serves as a dominant-negative inhibitor of the transcription factors C/EBP and LAP.
bacterially expressed CHOP inhibited the DNA-binding activity of C/EBP and LAP by forming heterodimers that could not bind DNA.Chop also activated Ero1-alpha (ERO1L), which encodes an ER oxidase.
Consequently, the ER of stressed Chop -/- cells was hypooxidized relative to the ER of stressed wildtype cells.
Pharmacologic and genetic manipulations promoting a hypooxidized ER reduced abnormal high molecular mass protein complexes and protected cells from the lethal consequences of ER stress.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse DDIT3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Ala168 of mouse DDIT3, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
