DOPA decarboxylase (EC 4.1.1.28) is an enzyme implicated in 2 metabolic pathways, synthesizing 2 important neurotransmitters, dopamine and serotonin.
Following the hydroxylation of tyrosine to form L-dihydroxyphenylalanine (L-DOPA), catalyzed by tyrosine hydroxylase (TH), DDC decarboxylates L-DOPA to form dopamine.
This neurotransmitter is found in different areas of the brain and is particularly abundant in basal ganglia.
Dopamine is also produced by DDC in the sympathetic nervous system and is the precursor of the catecholaminergic hormones, noradrenaline and adrenaline in the adrenal medulla.
In the nervous system, tryptophan hydroxylase produces 5-OH tryptophan, which is decarboxylated by DDC, giving rise to serotonin.
DDC is a homodimeric, pyridoxal phosphate-dependent enzyme.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human DDC.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Leu200-Asn420 of human DDC, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
