84-4414-08　DCT (Dopachrome Tautomerase, TYRP2, TRP-2, DT, Dopachrome Delta-Isomerase, Tyrosine-Related Protein 2, L-dopachrome Delta-isomerase) 200ul　362813

特徴

• Dopachrome tautomerase, also known as DCT, is an enzyme that isomerizes the melanogenic intermediate 3,4-dihydroxyphenylalanine-chrome (DOPAchrome) to 5,6-dihydroxyindole-2-carboxylic acid (DHICA) in the biosynthesis of melanin.
• Its expression is regulated by the Microphthalmia-associated transcription factor.
• Human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region.
• Northern blot analysis showed that TYRP2 is expressed at high levels in human melanoma cells.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher..
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VCM TVDSLVNKEC CPRLGAESAN VCGSQQGRGQ CTEVRADTRP WSGPYILRNQ DDRELWPRKF FHRTCKCTGN FAGYNCGDCK FGWTGPNCER KKPPVIRQNI HSLSPQEREQ FLGALDLAKK RVHPDYVITT QHWLGLLGPN GTQPQFANCS VYDFFVWLHY YSVRDTLLGP GRPYRAIDFS HQGPAFVTWH RYHLLCLERD LQRLIGNESF ALPYWNFATG RNECDVCTDQ LFG
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.