DCAMKL1 is a microtubule-associated kinase that can undergo autophosphorylation.
DCAMKL1 also has microtubule-polymerizing activity that is independent of its protein kinase activity.
The KIAA0369 cDNA encodes a 740aa peptide that shares 72.3%aa identity with doublecortin.
The protein has a doublecortin domain, a serine/proline-rich domain, and a calmodulin-dependent kinase-like domain.
The authors also identified 4 splice variants of KIAA0369.
All splice variants were expressed predominantly in fetal brain, with transcript sizes ranging from 8.0 to 5.0 kb.
Varying expression of all isoforms was also seen in all 15 regions of adult human brain examined, with greatest expression seen in cerebral cortex, occipital pole, frontal lobe, amygdala, and hippocampus.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human DCLK1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Tyr390-Val647 of human DCLK1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
