84-4413-98　DAPP1 (Dual Adaptor Of Phosphotyrosine And 3-Phosphoinositides, BAM32, B lymphocyte adapter protein Bam32, B-cell adapter molecule of 32kD, Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide) 200ul　362804

特徴

• Adaptor proteins are a link between 'upstream' cell surface receptors and nonreceptor protein tyrosine kinases and 'downstream' molecules such as phosphatidylinositol (PtdIns) 3-kinase (PI3K).Sequence analysis predicted that the 280aa protein contains a potential myristoylation site (a glycine after the N-terminal methionine), an N-terminal SH2 domain, and a C-terminal PH domain with a PtdIns-interacting motif.
• The authors noted that APS also has an SH2 domain and a PH domain but at opposite termini of the protein.
• PCR of cDNA libraries and Northern blot analysis revealed ubiquitous expression of 2.7-kb transcripts, with highest levels in placenta and lung.
• A protein-lipid overlay analysis indicated that the PH domain of DAPP1 interacts with physiologic enantiomers of PtdIns.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.