Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells.
These results suggest that DAPK3 may play a role in the induction of apoptosis.Saito et al.
(1998) systematically screened serine/threonine protein kinases by reverse transcription-PCR with degenerated primers corresponding to the conserved regions of the protein kinase domain.
One of the kinase genes identified showed a striking sequence homology to ZIP kinase reported by Kawai et al.
(1998).
Kawai et al.
(1998) reported that the ZIP kinase induced morphologic changes in apoptosis in mammalian cells when overexpressed, suggesting that it plays an important role in the induction of apoptosis.
The mRNA was ubiquitously expressed in various tissues.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse DAPK3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Arg448 of mouse DAPK3, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
