Database analysis showed that the SPECC1 gene is almost identical to the human sperm antigen, HCMOGT1.
There are 2 possible 5-prime ends and 2 possible 3-prime ends.
The major coding sequence shared by all isolated transcripts encodes an ATPase domain similar to that of SMC family members and a coiled-coil domain commonly seen in motor or structure proteins.
The larger transcript was also expressed in spleen, but all other normal tissues examined showed little or no SPECC1 expression.
Variable expression was detected in some human cancer cell lines, including HeLa cells.
Immunohistochemical analysis of HeLa cells detected SPECC1 in the nucleus.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse CYTSB.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Gly961-Lys1063 of mouse CYTSB, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
