Cysteine is an alpha-amino acid with the chemical formula HO2CCH(NH2)CH2SH.
It is a non-essential amino acid, which means that it is biosynthesized in humans.
Its codons are UGU and UGC.
The side chain on cysteine is thiol, which is nonpolar and thus cysteine is usually classified as a hydrophobic amino acid.
The thiol side chain often participates in enzymatic reactions, serving as a nucleophile.
The thiol is susceptible to oxidization to give the disulfide derivative cystine, which serves an important structural role in many proteins.
Cysteine is named after cystine.
The cysteine thiol group is nucleophilic and easily oxidized.
The reactivity is enhanced when the thiol is ionized, and cysteine residues in proteins have pKa values close to neutrality, so are often in their reactive thiolate form in the cell.
Because of its high reactivity, the thiol group of cysteine has numerous biological functions.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes CyS. Species Crossreactivity: pan-species (general)
