Cholesterol 7 alpha-hydroxylase (CYP7A1) is the rate-limiting enzyme in the synthesis of bile acid from cholesterol, catalyzing the formation of 7-alpha-hydroxycholesterol.
CYP7A1 is a cytochrome P450 heme enzyme that oxidizes cholesterol using molecular oxygen.
It is downregulated by Sterol regulatory element binding proteins (SREBP) when plasma cholesterol levels are low.
It is upregulated by the nuclear receptor LXR (liver X receptor) when cholesterol (specifically - oxysterol) levels are high.
The effect of this upregulation is to increase the production of bile acids and reduce the level of cholesterol in hepatocytes.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat CYP7A1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Phe109-Lys383 of rat CYP7A1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
