Cytochrome P450, family 11, subfamily A, polypeptide 1 (P45011a1), often referred to as P450scc is a mitochondrial enzyme associated with the conversion of cholesterol to pregnenolone.
The gene name is CYP11A1.
The term "desmolase" is becoming outdated and the enzyme is now commonly referred to as P450scc, where "scc" refers to side chain cleavage.
The protein is a member of the cytochrome family of enzymes.
The protein resides on the inner mitochondrial membrane, facing the interior (matrix).
The presence of this enzyme defines if a cell is steroidogenic.
Consequently, P450scc is found in all steroid-producing cell types such as theca cells and luteal cells in the ovary, Leydig cells in the testis and cell types in the adrenal cortex.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse CYP11A1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met329-Asn526 of mouse CYP11A1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
