Cytochrome c, or cyt c (horse heart: PDB 1HRC) is a small heme protein found loosely associated with the inner membrane of the mitochondrion.
It is a soluble protein, unlike other cytochromes, and is an essential component of the electron transfer chain.
It is capable of undergoing oxidation and reduction, but does not bind oxygen.
It transfers electrons between Complexes III and IV.
Cytochrome c is a highly conserved protein across the spectrum of species, found in plants, animals, and many unicellular organisms.
This, along with its small size (molecular weight about 12,000 daltons), makes it useful in studies of evolutionary divergence.
Its primary structure consists of a chain of 100aa.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse CYCS.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Gln13-Leu99 of mouse CYCS, fused to N-terminal His-Tag expressed in E. coli.
