CXADR is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses.
Pseudogenes of this gene are found on chromosomes 15, 18, and 21.
The deduced 365aa human peptide has a calculated molecular mass of 39kD and shares 83% sequence identity with the mouse homolog.
Both peptides contain a potential leader sequence, transmembrane domains, 2 N-linked glycosylation sites, and 2 extracellular disulfide-bonded loops resembling the characteristic V and C2 domains of the immunoglobulins.
Transfection of CXADR or Cxadr into NIH 3T3 cells conferred susceptibility to infection by CVB3 and CVB4, resulting in 100- to 1000-fold higher virus titers following exposure than that found in nontransfected control cultures.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CXADR.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Leu20-Val229 of human CXADR, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
