Connexin 43 is a connexin gap junction found in several organs and cell types, but is most often found in the heart.
Current data suggest Cx43 is essential for the development of normal cardiac architecture and ventricular conduction.
Two members of the connexin gene family, connexins 43 and 32, or GJB1, are abundantly expressed in the heart and liver, respectively.
GAP43-like immunoreactivity in rat is mainly present in sympathetic and sensory nerve fibers as well as in perivascular nerve terminals.
This peptide is axonally transported predominantly in sensory and adrenergic axonsSohl et al.
(2003) stated that the mouse and human CX43 share 97% aa identity.
Northern blot analysis detected variable expression of a 3.0-kb CX43 transcript in both mouse and human, with highest expression in heart.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:50-100Optimal dilutions to be determined by the researcher.
AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- S LSAVYTCKRD PCPHQVDCFL SRPTEKTIFI IFMLVVSLVS LALNIIELFY VFFKGVKDRV KGKSDPYHAT SGALSPAKDC GSQKYAYFNG CSSPTAPLSP MSPPGYKLVT GDRNNSSCRN YNKQASEQNW ANYSAEQNRM GQAGSTISNS HAQPFDFPDD NQNSKKLAAG HELQPLAIVD QRPSSRASSR ASSRPRPDDL EI
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CX43.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser180-Ile382 of human CX43, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
