Gap junction alpha-4 protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell.
Mutations in this gene have been associated with atherosclerosis and a higher risk of myocardial infarction.
Reed et al.
(1993) used PCR amplification and cDNA library screening to clone DNA encoding the connexin 37 gap junction protein.
The derived polypeptide contained 333aa, with a predicted molecular mass of about 37kD.
RNA blots demonstrated that CX37 is expressed in multiple organs and tissues, including heart, uterus, ovary, and blood vessel endothelium, and in primary cultures of vascular endothelial cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse CX37.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Thr19-Asp245 of mouse CX37, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
