Cullin 1 is a protein plays an important role in protein degradation and protein ubiquitination.
This is an essential component of the SCF E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.
In the SCF complex, it serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits.
May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.
This protein is a part of a SCF complex consisting of CUL1, RBX1, SKP1 and SKP2.
It also interacts with RNF7.
Part of a complex with TIP120A/CAND1 and RBX1.
The unneddylated form interacts with TIP120A/CAND1 and the interaction negatively regulates the association with SKP1 in the SCF complex.
Interacts with COPS2.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CUL1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Glu250 of human CUL1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
