Cathepsin A is a ubiquitously expressed multifunctional enzyme, with deamidase, esterase, and carboxypeptidase activities and a preference for substrates with hydrophobic amino acid residues at the P1-prime position.
Association with CTSA, as part of the lysosomal multienzyme complex, is essential for stabilization of lysosomal beta-galactosidase (GLB1), as well as for activation of the lysosomal neuraminidase (NEU1).
Galjart et al.
isolated cDNA encoding the human 'protective protein.' The cDNA directed the synthesis of a 452aa precursor molecule that is processed in vivo to yield mature 'protective protein,' a heterodimer of 32kD and 20kD polypeptides held together by disulfide bridges.
Because of homology to yeast carboxypeptidase Y, Galjart et al.
suggested a protease activity for the 'protective protein.'
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes bovine CTSA.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Bo
Immunogen：Recombinant protein corresponding to Leu171-Lys417 of bovine CTSA, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
