Alpha-chymotrypsin is one of a family of serine proteases secreted into the gastrointestinal tract as the inactive precursor chymotrypsinogen.
The zymogen is activated by proteolytic cleavage by trypsin (PRSS1).
Homology indicates that CTRB is on 16q because it is one of 7 genes that are on 16 in man and on chromosome 8 in the mouse; the other 6 are on 16q, whereas human 16p, which carries the alpha-globin gene cluster, appears to be homologous to mouse 11.
The coding region contains 789 bp.
The predicted product consisted of 263aa, including 18aa for a signal peptide.
Southern blot analyses using the cloned cDNA as a probe showed that human genomic DNA carries at least 2 genes that are related to chymotrypsinogen.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CTRB.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ile34-Ala261 of human CTRB1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
