In adherens junctions, alpha-catenin links the cadherin/beta-catenin complex to the actin-based cytoskeleton.
Alpha-catenin is a homodimer in solution, but forms a 1:1 heterodimer with beta-catenin.
Pokutta and Weis (2000) determined the crystal structure of the alpha-catenin dimerization domain, residues 82 to 279.
The crystal structure showed that alpha-catenin dimerizes through formation of a 4-helix bundle in which 2 antiparallel helices are contributed by each protomer.
A slightly larger fragment, containing residues 57 to 264, bound to beta-catenin.
The crystal structure of a chimera consisting of the alpha-catenin-binding region of beta-catenin linked to the N terminus of alpha-catenin residues 57 to 264 revealed the interaction between alpha- and beta-catenin and provided a basis for understanding adherens junction assembly.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CTNNa1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Thr2-Cys228 of human CTNNa1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
