Using an expression cloning approach, Wille et al.
(2001) obtained a cDNA encoding CDw92.
The deduced 652aa CDw92 protein is 99% identical to the CTL1 protein reported by O'Regan et al.
(2000).
CDw92 has cytoplasmic N and C termini, 3 potential N-glycosylation sites, and a potential ITIM (immunoglobulin tyrosine-based inhibitory motif).
Immunoprecipitation analysis showed that CDw92 is expressed as a 70kD protein.
Flow cytometric analysis demonstrated expression of CDw92 on almost all B lymphocytes, most T lymphocytes, some NK cells, and some myeloid cell lines tested.
Maturation of monocyte/dendritic cells resulted in downregulation of CDw92, and expression could subsequently be upregulated by interleukin-10 (IL10).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CTL1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Thr74-Cys183 of human CTL1, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
