Chitobiase is a lysosomal glycosidase involved in degradation of asparagine-linked oligosaccharides on glycoproteins.
There was a fused in-frame sequence that represented exons 2 and 3 from the gene for the G protein gamma-5 subunit (GNG5).
Exons 2 and 3, respectively, code for the carboxyl-end of the gamma-5 protein and the 3-prime untranslated portion of its mRNA.
This hybrid message had also been identified in human liver and retina by RT-PCR analysis.
It was not known whether a hybrid protein was produced in vivo.
Ahmad et al.
(1995) demonstrated that both the CTB and the GNG5 genes map to 1p by PCR analysis of somatic cell hybrids; by fluorescence in situ hybridization using a YAC clone which contained both genes, they refined the localization to 1p22.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CTBS.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu47-Arg385 of human CTBS fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
