The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences.
Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity.
There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens.
The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions.
The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes
CST5 is located in the cystatin locus and encodes a protein found in saliva and tears.
The encoded protein may play a protective role against proteinases present in the oral cavity.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CST5.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human CST5, Gly21-Val142 of human CST5, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
