Chondroitin sulfate proteoglycan 5 is a protein encoded by the CSPG5 gene.Yasuda et al.
(1998) cloned CSPG5 from a human brain cDNA library, using a rat cDNA homolog as probe.
The deduced 539aa protein has a multidomain structure consisting of an N-terminal signal sequence, a chondroitin sulfate-attachment domain, an acidic aa cluster, an EGF-like domain, a transmembrane domain, and a cytoplasmic tail.
The human and rat proteins share 86% homology.
Northern blot analysis detected restricted expression of a 2.4-kb transcript in brain.
A faint 5.2-kb band was detected in heart.
Neuroglycan C (NGC) is a transmembrane chondroitin sulfate proteoglycan expressed predominantly in the brain that possesses an EGF-like extracellular domain.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat CSPG5.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Ala139-Arg370 from rat CSPG5, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
