KCA gene extends over 8,821 nucleotides and consists of 5 exons ranging from 33 to 496 nucleotides separated by introns ranging from 1,146 to 2,942 nucleotides.
The human and bovine KCA genes are very similar, with a conserved gene organization and observed identities of 81% and 87% for exons 1 and 2, respectively.
The human alpha-S1-casein, beta-casein, and kappa-casein genes are closely linked and arranged in that order.
The distance between the alpha-S1 and beta and between alpha-S1 and kappa is approximately 10 and 300 kb, respectively.
The beta-casein gene is oriented in the opposite direction to the alpha-S1 and kappa genes.
By fluorescence in situ hybridization, the casein gene family was localized to 4q21.1.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C.
for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes human CSN3.
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to Asn24-Thr181 of human CSN3, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.