Milk casein can be separated by urea starch electrophoresis into 3 regions, alpha, beta, and kappa casein.
Alpha and beta variants are present in the human population.
In Italy the frequency of the 2 alpha alleles was 0.908 and 0.092; 2 beta alleles had a frequency of 0.678 and 0.322.
The human alpha-S1, beta-, and kappa-casein genes are closely linked and arranged in that order.
By fluorescence in situ hybridization, they demonstrated that the casein gene family is localized to 4q21.1.
the human 'locus' comprises at least 4 casein genes: 3 genes encoding calcium-sensitive, casein-like genes, and 1 kappa-casein gene, in the order alpha-s1--beta--alpha-s2--kappa.
The approximate size of the human casein gene locus is 350 kb.
the casein cluster is located within 700 kb of the albumin gene cluster, which is located on 4q13.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C.
for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CSN1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Arg16-Trp185 of human CSN1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
