CSE is an enzyme which breaks down cystathionine into cysteine and alpha-ketobutyrate.
Pyridoxal phosphate is a prosthetic group of this enzyme.
The bioavailability of cysteine is rate-limiting for the hepatic synthesis of glutathione.
Cysteine is provided through either the diet or the transsulfuration pathway, in which cystathionine derived from methionine is transformed into cysteine by cystathionine gamma-lyase.Mutant mice lacking CTH displayed pronounced hypertension and diminished endothelium-dependent vasorelaxation.
Cystathionine was physiologically activated by calcium and calmodulin, which is a mechanism for H2S formation in response to vascular activation.
Yang et al.
(2008) concluded that their findings provided direct evidence that H2S is a physiologic vasodilator and regulator of blood pressure.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C.
for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat CSE.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Val36-His299 of rat CSE, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
