RNase protection assays showed that this gene, termed UNR by them, is transcribed in the same direction as NRAS and that its 3-prime end is located only 130 bp from the transcription initiation site of NRAS.
The close spatial relationship was conserved in all species from which the NRAS gene had been isolated.
The UNR cDNA contained an open reading frame capable of encoding a protein of 798aa.
UNR transcripts were detected in mouse, rat, and human cells.
Only a single copy of UNR was detected in the mouse.
The gene produced multiple transcripts that differed in their 3-prime ends and apparently resulted from the differential use of multiple polyadenylation sites located in the 3-prime untranslated region of the gene.
Both UNR and NRAS were expressed in all tissues examined and the 2 genes may be coordinately regulated.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C.
for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CSDE1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Val245 from human CSDE1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
