CRY proteins belong to a superfamily of flavoproteins that occurs in all kingdoms of life.
The structure has a fold very similar to photolyase, with a single molecule of FAD noncovalently bound to the protein.
These proteins have variable lengths and surfaces on the C-terminal end when compared to one another, due to the lack of DNA repair enzymes which ultimately results in changes in genome and appearance.
The Ramachandran plot, shows that the secondary structure of the CRY1 protein is primarily in a right-handed alpha helix conformation with little to no steric overlap.
The structure of CRY1 is almost entirely made up of alpha helicies, with remaining portions in loops and has few beta sheets present in the molecule.
The molecule is arranged as an orthogonal bundle.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CRY1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Val3-Leu132 of human CRY1, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
