CRTAP is similar to the chicken and mouse CRTAP genes.
The encoded protein is a scaffolding protein that may influence the activity of at least one member of the cytohesin/ARNO family in response to specific cellular stimuli.
Crtap is highly expressed in mouse growth plate proliferating chondrocytes and in cells at the chondroosseous junction.
At embryonic day 15.5, it is expressed strongly in the presumptive bone collar of the diaphyses, where vascular and osteoblast invasion is initiated.
Its expression is low in hypertrophic chondrocytes, and RT-PCR analysis confirmed expression in both osteoclasts and osteoblasts.
Most of the Crtap protein is located within cells, although some signal was detected in the extracellular matrix.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse CRTAP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Gln26-Ala400 of mouse CRTAP, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
