Calreticulin is a protein that binds Ca2 ions (a second messenger molecule in signal transduction), rendering it inactive.
The Ca2 is bound with low affinity, but high capacity, and can be released on a signal.
Calreticulin is located in storage compartments associated with the endoplasmic reticulum.
Calreticulin is also known as calregulin, CRP55, CaBP3 and calsequestrin-like protein.
The term "Mobilferrin" is considered to be the same as calreticulin by some sources.Calreticulin binds to misfolded proteins and prevents them from being exported from the Endoplasmic reticulum to the Golgi apparatus.
A similar quality control chaperone, calnexin, performs the same service for soluble proteins as does calreticulin.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse CRT.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Asp199-Tyr308 of mouse CRT, fused to N-terminal His-Tag expressed in E. coli.
