In the course of characterizing a large number of genomic clones isolated from mouse chromosome 17, Kasahara et al.
(1987) found a cosmid clone containing a sequence transcribed specifically in the testis.
Subsequent studies (Kasahara et al., 1989) demonstrated a map location between Pgk2 and Mep1 on mouse chromosome 17, making it unlikely that the gene is involved in t mutant phenotypes.
Kasahara et al.
(1989) described the cDNA sequences of the mouse gene, named Tpx1, and of its human counterpart TPX1.
The 2 showed 77.8% nucleotide and 70% aa sequence similarity.
The mouse gene showed 64.2% nucleotide and 55.1% aa sequence similarity to that of the rat sperm-coating glycoprotein gene (Scg), the protein product of which is secreted by the epididymis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.5
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CRISP2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Lys22-Asn240 of human CRISP2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
