Corticotropin-releasing factor-binding protein is a protein encoded by the CRHBP gene.
Corticotropin-releasing hormone is a potent stimulator of synthesis and secretion of preopiomelanocortin-derived peptides.
Although CRH concentrations in the human peripheral circulation are normally low, they increase throughout pregnancy and fall rapidly after parturition
Potter et al.
(1991) isolated and partially purified the binding protein and cloned and characterized its cDNA from human liver and rat brain.
Expression of the cDNAs in COS-7 cells showed that the protein binds CRH with the same affinity as the native protein.
Furthermore, the recombinant binding protein inhibited CRH binding to a CRH antibody and inhibited CRH-induced release of ACTH by pituitary cells in vitro.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat CRHBP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Tyr25-Leu322 of rat CRHBP, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
