Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated.
The B1 and B2 forms differ from each other mainly in isoelectric point.
Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants.
It is not elevated in pancreatic carcinoma
The deduced 416aa preproenzyme (EC 3.4.17.2) has a 15aa signal peptide and a 95aa activation peptide.
It shares 76% and 84% sequence identity with the rat and bovine homologs, respectively.
DNA blot analysis indicated that the PCPB mRNA is transcribed from a single locus.
Northern blot analysis demonstrated pancreas-specific expression.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat CPB1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Trp120-Tyr415 of rat CPB1, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
