Carboxypeptidase A4 is a member of the carboxypeptidase A/B subfamily, and it is located in a cluster with three other family members on chromosome 7.
Carboxypeptidases are zinc-containing exopeptidases that catalyze the release of carboxy-terminal aa and are synthesized as zymogens that are activated by proteolytic cleavage.
This gene could be involved in the histone hyperacetylation pathway.
It is imprinted and may be a strong candidate gene for prostate cancer aggressiveness.
The deduced 421aa CPA4 protein contains 2 zinc-binding signature domains of metallocarboxypeptidases and a 16aa N-terminal signal peptide.
The protein shares 63% and 61% sequence identity with human and rat CPA2, respectively.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CPA4.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Lys55-Tyr421 of human CPA4, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
