The enzyme now called CPA3 was originally named mast cell carboxypeptidase A, and another protein was initially called CPA3.
A gene nomenclature committee renamed mast cell carboxypeptidase A as CPA3, and the original CPA3 reported by Huang et al became CPA4 to reflect the order of their discovery.
CPA3 is secreted from mast cells and has a pH optimum in the neutral to basic range.
It resembles pancreatic carboxypeptidases A1 in cleaving COOH-terminal aromatic and aliphatic aaresidues.
CPA3 functions together with endopeptidases secreted from mast cells such as chymases and tryptases to degrade proteins and peptides.
Upon mast cell activation and degranulation, CPA3, the chymases, and tryptases are released in complexes with heparin proteoglycan.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse CPA3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ile16-Ser417 of mouse CPA3, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
