Three different forms of human pancreatic procarboxypeptidase A have been isolated.
The A1 and A2 forms are monomeric proteins with different biochemical properties.
The A2 form of pancreatic procarboxypeptidase acts on aromatic C-terminal residues.
The full-length cDNA encodes a deduced 417aa protein, which includes a short leader sequence and a 94aa long activation segment.
The human CPA2 protein shares 89% similarity with the rat CPA2 protein and 64% similarity with human CPA1.
The A2 form of pancreatic procarboxypeptidase acts on aromatic C-terminal residues, in contrast to the A1 form, which shows a preference for the aliphatic C-terminal residues of peptide substrates.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat CPA2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Leu26-Tyr417 of rat CPA2, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
