Carboxypeptidase A1 is a monomeric pancreatic exopeptidase.
It is involved in zymogen inhibition.
The method allowed the quantitative and simultaneous analysis of a large number of transcripts.
To demonstrate the strategy, short diagnostic sequence tags (SSTs) were isolated from pancreas, concatenated, and cloned.
Manual sequencing of 1,000 tags revealed a gene expression pattern characteristic of pancreas.
New pancreatic transcripts corresponding to novel tags were also identified.
SAGE is based on 2 principles: first, that a short nucleotide sequence tag (9 to 10 bp) contained sufficient information to uniquely identify a transcript; and second, that concatenation of SSTs allows the efficient analysis of transcripts in a serial manner by the sequencing of multiple tags within a single clone.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat CPA1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Thr205-Tyr419 of rat CPA1, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
