The deduced 461aa CORO1A protein shares 40%aa identity with coronin, an actin-binding protein of Dictyostelium discoideum.
Sequence analysis predicted that CORO1A contains a WD (trp/asp) repeat and a leucine zipper motif.
Immunoblot analysis revealed cross-reactivity of human, calf, and mouse CORO1A, and Coro1a was clearly expressed in mouse brain, thymus, spleen, bone marrow, and lymph node, with weak expression in lung and gut, and no expression in liver, kidney, stomach, and skeletal muscle.
CORO1A suppressed Toll-like receptor (TLR) signaling following expression in a human monocytic cell line.
TLR2-mediated activation of the innate immune response resulted in suppression of CORO1A expression.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat CORO1A.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Met1-Glu204 of rat CORO1A, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
