Cartilage oligomeric matrix protein (COMP) is a member of the thrombospondin family of extracellular matrix proteins.
The protein consists of five 87kD subunits held together by interchain disulfide bonds, forming a 435kD pentameric protein.
COMP is expressed in all types of cartilage, vitreous of the eye, tendon, and vascular smooth muscle cells
Immunohistological staining of articular cartilage has revealed a developmentally regulated localization of COMP to the chondrocyte territorial and interterritorial matrix.
COMP binds in a zinc-dependent manner to collagen type I and type II and also to collagen type IX.
The thrombospondin family currently includes five members: TSP-1, TSP-2, TSP-3, TSP-4, and COMP (also referred to as TSP-5).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry (Paraffn): 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Positive Control Included:362639A: COMP, Positive ControlSuppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 50mM DTT, 0.02% BPB, 0.02% sodium azide, 50% glycerol)
DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
Stable for 6 months after receipt.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Specificity：Recognizes human COMP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser123-His240 of human COMP expressed in E. coli.
