Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits.
These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen.
Collagen alpha-3(IV) chain.
In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli.
The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein.
A specific kinase phosphorylates aa in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
