84-4412-21　COL1a2 (Collagen Type I Alpha 2, COL1-A2, OI4, Collagen Alpha-2(I)chain, osteogenesis imperfecta type IV, Collagen Of Skin,Tendon And bone,Alpha-2 Chain) 200ul　362628

特徴

• Collagen alpha-2(I) chain is a protein, one of the chains for type I collagen, the fibrillar collagen found in most connective tissues.
• Mutations in this gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome.
• De Wet et al.
• (1987) isolated 60 kb of cloned DNA containing the entire COL1A2 gene and 22 kb of flanking sequences.
• Like the homologous avian gene, the 1,366aa residues of the human prepropolypeptide chain are encoded by 52 exons.
• Analysis of the 5-prime and 3-prime untranslated regions conclusively established the nature of 5 polymorphic mRNA transcripts.
• The exons are equally distributed as follows: 6 in the N-propeptide domain, 42 in the alpha-chain region, and 4 in the C-propeptide domain.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.